Spectrophotometric detection and quantification limits of fipronil and neonicotinoids in acetonitrile

Riad Fouad, Mohamed

doi:10.21608/ijfsnh.2022.254720

Spectrophotometric detection and quantification limits of fipronil and neonicotinoids in acetonitrile

Document Type : Original Research

Author

Mohamed Riad Fouad

Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University

10.21608/ijfsnh.2022.254720

Abstract

Spectrophotometer is a commonly used and basic analytical technique
that can be used for both quantitative and qualitative examination in
chemical, pesticides and other materials. In this work, it was studied that
spectral-density (S-D) curve, calibration density (C-D) curve and
quantification limits for fipronil and neonicotinoids insecticides in
acetonitrile solvent on UV-Vis Spectrophotometer (Thermo Corporation,
Nicolet, evolution 100) were maximum absorption (λ max) equal 208, 256,
269 and 246 nm for fipronil, thiamethoxam, imidacloprid and
acetamiprid, respectively. The slope and R2 were 0.059 and 0.9545 for
fipronil, 0.044 and 0.9989 for thiamethoxam, 0.046 and 0.999 for
imidacloprid and 0.249 and 0.9996 for acetamiprid, respectively. The
limit of blank (LOB), limits of detection (LOD), and quantification
(LOQ) were found to be 0.0614, 0.0623 and 0.906 μg mL-1 for fipronil,
0.0613, 0.062 and 0.0865 μg mL-1 for thiamethoxam and 0.0946, 0.954
and 0.123 μg mL-1 for imidacloprid and 0.0478, 0.0489 and 0.158 μg
mL-1 for acetamiprid, respectively.

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